Monday, 4 September 2017

Advanced Protein Engineering for Rapid MAb Purification

Protein engineering focuses on changing the sequence and structure of protein to produce enzymes with enhanced functional properties. It can enhance a specific activity, stability, and selectivity of protein toward non-natural substrates. This mainly depends on the applications of recombinant DNA technology to modify amino acid sequences. Latest advancements in protein engineering have transformed the production of commercially available enzymes into enhanced industrial catalysts. Constant efforts by various regulatory authorities to decrease the time & cost associated with drug discovery can further revolutionize biological sciences.


MAb Purification with Protein Capture

Recently, W. L. Gore & Associates, Inc. launched their new “Protein Capture Devices” product line. It contains Protein A, designed for novel drug discovery and research application areas. Protein A can fundamentally enhance purification of monoclonal antibodies (MAb).

How It Works?

This device utilizes an exclusive expanded polytetrafluoroethylene (ePTFE) membrane composite. This composite offers binding capability at high flow rates and enhances the speed of purification process. At present, these membrane devices are available in 1.0 mL (= 30 mg) and 3.5 mL (= 105 mg) capacity.Unlike other resin-based columns, these devices can line possess higher binding capacity at a low residence time. This can allow faster processing time and possibility of highly concentrated elution pools. This can enable the researchers to monitor more candidates in a short span. They can potentially eradicate a downstream concentration stage during the process of antibody purification.

Market Insights

According to Grand View Research, Inc.; the global protein engineering market is anticipated to witness substantial growth during the forecast period (from 2014 to 2025). High preference for protein therapeutics over non-protein drugs can drive the growth in the near future. 

Market Competitors

Bruker Corporation; GE Healthcare; Sigma-Aldrich Corporation; Waters Corporation; Danaher Corporation; and Perkinelmer, Inc. are some of the prominent players operating in the worldwide market. Most companies focus on novel product development initiatives, geographical expansions, and mergers & acquisitions to maintain dominant positions in the global market.

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